Illumina Sequencing Methods

Accurate total NGS solutions including library preparation, sequencing systems, and data analysis

Key Sequencing Methods

DNA Sequencing

Discover the entire genome with whole-genome solutions, or focus on specific regions of interest with whole-exome and targeted sequencing options.

RNA Sequencing

Take advantage of a broad range of sequencing techniques for targeted RNA or whole-transcriptome studies.

Methylation Sequencing

Enhance epigenetic studies with high-coverage density and flexibility enabled by sequencing-based DNA methylation analysis.


Illumina Next-Generation Sequencing Platforms

Illumina offers innovative next-generation sequencing (NGS) platforms that deliver industry-leading data quality and accuracy, at an unparalleled scale. With flexible throughput and simple, streamlined workflows, our sequencing platforms are transforming the field of genomics.

Illumina technology empowers more and more NGS users every day. NGS has transformed the genomics community to include clinical, agrigenomics, and forensic genomics researchers, and continues to enable advances in cancer genomics, microbiology, and other areas.

Find the sequencing system that's right for your lab

NGS Platforms with Proven Performance

Illumina NGS platforms have been adopted by leading institutions around the world, both big and small, and are the production platform for all major genome centers. More bases have been sequenced using Illumina chemistry than all other next-generation sequencing platforms combined.

Our newly expanded sequencing platform portfolio will transform your research and enable new discoveries.

Illumina systems deliver what you need, when you need it.

Each platform delivers industry-leading data quality and performance, with flexible throughput and simple, streamlined workflows—for every kind of researcher, for every scale of study.

Next-Generation Sequencing Platform Innovation is in our DNA

Illumina has pioneered major advances for NGS platforms in simplicity and flexibility – all while delivering industry-leading performance. Sequencing output has grown a thousand-fold, from less than 1 gigabase to over 1 terabase per run, while the number of reads has increased from millions to billions.

Experiments that once required complex workflows now use simple push-button sequencing. Illumina continues to lead the field in sequencing platform innovation and performance.

Library preparation

Introduction to Library Preparation

A key element of Illumina next-generation sequencing (NGS) is high-quality library preparation. Illumina library prep protocols can accommodate a range of throughput needs, from lower-throughput protocols for small laboratories to fully automated library preparation workstations for large laboratories or genome centers.

Illumina offers comprehensive sequencing library preparation solutions for a wide variety of NGS methods, including whole-genome sequencing, targeted DNA sequencing, whole-transcriptome sequencing, targeted RNA sequencing, and more. These solutions support a variety of sample types, from cell lines to fresh tissue, formalin-fixed paraffin-embedded (FFPE) samples, blood, and other challenging sample types.

Kits for NGS Library Preparation

Illumina offers an extensive assortment of easy-to-use next-gen sequencing library preparation kits for DNA, RNA, and epigenetic sequencing studies. To find the right kit for your needs, use the selection tool below, or view a filterable list of library prep kits.

Walk-Away NGS Library Prep

The NeoPrep Library Prep System radically simplifies preparation of next-generation sequencing libraries by significantly decreasing the number of steps, improving reproducibility, and greatly reducing hands-on time. The system performs PCR amplification, quantification, and normalization, generating up to 16 sequencing-ready libraries per run. NeoPrep Library Prep System

High-Throughput Automation Solutions

For labs preparing large quantities of NGS libraries, liquid-handling robots and additional automation solutions provide high-throughput capacity and important quality control touch points. Visit the page below to learn more about Illumina-qualified sequencing library prep automation methods available through our partners. Methods for library prep automation

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